A New Genotyping Method for Detecting Low Abundance Single Nucleotide Mutations Based on Gap Ligase...
A New Genotyping Method for Detecting Low Abundance Single Nucleotide Mutations Based on Gap Ligase Chain Reaction and Quantitative PCR Assay
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Author / Creator
Yi, Ping , Jiang, Hongmei , Li, Li , Dai, Faguo , Zheng, Yingru , Han, Jian , Chen, Zhuqin and Guo, Jianxin
Publisher
New York: Humana Press Inc
Journal title
Language
English
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Publication information
Publisher
New York: Humana Press Inc
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Scope and Contents
Contents
We tested applicability of a new genotyping technique to detect a low abundance CD17 (A → T) mutation of β-globin gene. The technique utilized a combined gap ligase chain reaction (Gap-LCR) and quantitative PCR (qPCR) methods. One pair of Gap-LCR primers was modified by adding specific sequences to the 5′ end of the upstream and the 3′ end of the d...
Alternative Titles
Full title
A New Genotyping Method for Detecting Low Abundance Single Nucleotide Mutations Based on Gap Ligase Chain Reaction and Quantitative PCR Assay
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Author / Creator
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Record Identifier
TN_cdi_proquest_miscellaneous_915632885
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_915632885
Other Identifiers
ISSN
1085-9195
E-ISSN
1559-0283
DOI
10.1007/s12013-011-9277-2
