Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
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Publisher
New York: Nature Publishing Group US
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Language
English
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Publisher
New York: Nature Publishing Group US
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In vivo
calcium imaging at multiple depths simultaneously is shown using multifocal two-photon microscopy and spatiotemporal multiplexing. This technique involves scanning the sample with multiple beams in parallel at different axial planes and is applied to monitor neuronal network activity in multiple cortical layers of an anesthetized mouse.<...
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Full title
Simultaneous two-photon calcium imaging at different depths with spatiotemporal multiplexing
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TN_cdi_proquest_miscellaneous_918044170
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_proquest_miscellaneous_918044170
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.1552