Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA pr...
Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing
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New York: Nature Publishing Group US
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English
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New York: Nature Publishing Group US
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Contents
Non-cleaving Cas9 (dCas9) is widely employed to manipulate specific gene loci, often with scant regard for unintended transcriptional effects. We demonstrate here that dCas9 mediates precise RNA polymerase II transcriptional pausing followed by transcription termination and potential alternative polyadenylation. By contrast, alternative splicing is...
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Full title
Elongation roadblocks mediated by dCas9 across human genes modulate transcription and nascent RNA processing
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10584677
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_10584677
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ISSN
1545-9993
E-ISSN
1545-9985
DOI
10.1038/s41594-023-01090-9
