Targeted barcoding of variable antibody domains and individual transcriptomes of the human B-cell re...
Targeted barcoding of variable antibody domains and individual transcriptomes of the human B-cell repertoire using Link-Seq
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Publisher
England: Oxford University Press
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Language
English
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Publisher
England: Oxford University Press
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Contents
Here, we present Link-Seq, a highly efficient droplet microfluidic method for combined sequencing of antibody-encoding genes and the transcriptome of individual B cells at large scale. The method is based on 3' barcoding of the transcriptome and subsequent single-molecule PCR in droplets, which freely shift the barcode along specific gene regions,...
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Full title
Targeted barcoding of variable antibody domains and individual transcriptomes of the human B-cell repertoire using Link-Seq
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11759286
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_11759286
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ISSN
2752-6542
E-ISSN
2752-6542
DOI
10.1093/pnasnexus/pgaf006
