Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus
Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus
About this item
Full title
Author / Creator
Publisher
United States: Elsevier Inc
Journal title
Language
English
Formats
Publication information
Publisher
United States: Elsevier Inc
Subjects
More information
Scope and Contents
Contents
We constructed a “temperature-jump/stopped-flow” apparatus that allows us to study fast enzyme reactions at extremely high temperatures. This apparatus is a redesigned stopped-flow which is capable of mixing the reactants on a submillisecond timescale concomitant with a temperature-jump even as large as 60°C. We show that enzyme reactions that are...
Alternative Titles
Full title
Enzyme Kinetics above Denaturation Temperature: A Temperature-Jump/Stopped-Flow Apparatus
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1779911
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_1779911
Other Identifiers
ISSN
0006-3495
E-ISSN
1542-0086
DOI
10.1529/biophysj.106.092833
