Optical Lock-In Detection of FRET Using Synthetic and Genetically Encoded Optical Switches
Optical Lock-In Detection of FRET Using Synthetic and Genetically Encoded Optical Switches
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United States: Elsevier Inc
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English
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United States: Elsevier Inc
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The Förster resonance energy transfer (FRET) technique is widely used for studying protein interactions within live cells. The effectiveness and sensitivity of determining FRET, however, can be reduced by photobleaching, cross talk, autofluorescence, and unlabeled, endogenous proteins. We present a FRET imaging method using an optical switch probe,...
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Full title
Optical Lock-In Detection of FRET Using Synthetic and Genetically Encoded Optical Switches
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2480664
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2480664
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ISSN
0006-3495,1542-0086
E-ISSN
1542-0086
DOI
10.1529/biophysj.107.124859
