Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach
Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
This protocol provides a method for quantitating the intracellular concentrations of endogenous metabolites in cultured cells. The cells are grown in stable isotope-labeled media to near-complete isotopic enrichment and then extracted in organic solvent containing unlabeled internal standards in known concentrations. The ratio of endogenous metabol...
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Full title
Absolute quantitation of intracellular metabolite concentrations by an isotope ratio-based approach
Authors, Artists and Contributors
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2710577
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2710577
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2008.107
