Temperature-Sensitive Mutations Made Easy: Generating Conditional Mutations by Using Temperature-Sen...
Temperature-Sensitive Mutations Made Easy: Generating Conditional Mutations by Using Temperature-Sensitive Inteins That Function Within Different Temperature Ranges
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Publisher
United States: Genetics Soc America
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Language
English
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United States: Genetics Soc America
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Contents
Reversible and easy to use, temperature-sensitive (TS) mutations are powerful tools for studying gene function. However, TS alleles are rare and difficult to generate and identify, and this has limited their use in most multicellular organisms. We have generated and characterized 41 intein switches, temperature-sensitive Sce VMA mutations that spli...
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Full title
Temperature-Sensitive Mutations Made Easy: Generating Conditional Mutations by Using Temperature-Sensitive Inteins That Function Within Different Temperature Ranges
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2746138
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2746138
Other Identifiers
ISSN
0016-6731,1943-2631
E-ISSN
1943-2631
DOI
10.1534/genetics.109.104794
