Multicentre validation study of nucleic acids extraction from FFPE tissues
Multicentre validation study of nucleic acids extraction from FFPE tissues
About this item
Full title
Author / Creator
Bonin, Serena , Hlubek, Falk , Benhattar, Jean , Denkert, Carsten , Dietel, Manfred , Fernandez, Pedro L. , Höfler, Gerald , Kothmaier, Hannelore , Kruslin, Bozo , Mazzanti, Chiara Maria , Perren, Aurel , Popper, Helmuth , Scarpa, Aldo , Soares, Paula , Stanta, Giorgio and Groenen, Patricia J. T. A.
Publisher
Berlin/Heidelberg: Springer-Verlag
Journal title
Language
English
Formats
Publication information
Publisher
Berlin/Heidelberg: Springer-Verlag
Subjects
More information
Scope and Contents
Contents
In most pathology laboratories worldwide, formalin-fixed paraffin embedded (FFPE) samples are the only tissue specimens available for routine diagnostics. Although commercial kits for diagnostic molecular pathology testing are becoming available, most of the current diagnostic tests are laboratory-based assays. Thus, there is a need for standardized procedures in molecular pathology, starting from the extraction of nucleic acids. To evaluate the current methods for extracting nucleic acids from FFPE tissues, 13 European laboratories, participating to the European FP6 program IMPACTS (
www.impactsnetwork.eu
), isolated nucleic acids from four diagnostic FFPE tissues using their routine methods, followed by quality assessment. The DNA-extraction protocols ranged from homemade protocols to commercial kits. Except for one homemade protocol, the majority gave comparable results in terms of the quality of the extracted DNA measured by the ability t...
Alternative Titles
Full title
Multicentre validation study of nucleic acids extraction from FFPE tissues
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2933807
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_2933807
Other Identifiers
ISSN
0945-6317
E-ISSN
1432-2307
DOI
10.1007/s00428-010-0917-5
