Insertion of the Designed Helical Linker Led to Increased Expression of Tf-Based Fusion Proteins
Insertion of the Designed Helical Linker Led to Increased Expression of Tf-Based Fusion Proteins
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Publisher
Boston: Boston : Springer US
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Language
English
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Boston: Boston : Springer US
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Purpose To demonstrate a high-level expression of transferrin (Tf)-based fusion proteins by inserting a helical linker between two protein domains. Methods Tf-based fusion proteins were designed to contain oligonucleotides encoding a helical linker inserted between the protein domains. Plasmid constructs were transfected into HEK293 cells and the s...
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Full title
Insertion of the Designed Helical Linker Led to Increased Expression of Tf-Based Fusion Proteins
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3121561
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3121561
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ISSN
0724-8741
E-ISSN
1573-904X
DOI
10.1007/s11095-008-9767-0
