Genetic engineering of human ES and iPS cells using TALE nucleases
Genetic engineering of human ES and iPS cells using TALE nucleases
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Author / Creator
Hockemeyer, Dirk , Wang, Haoyi , Kiani, Samira , Lai, Christine S , Gao, Qing , Cassady, John P , Cost, Gregory J , Zhang, Lei , Santiago, Yolanda , Miller, Jeffrey C , Zeitler, Bryan , Cherone, Jennifer M , Meng, Xiangdong , Hinkley, Sarah J , Rebar, Edward J , Gregory, Philip D , Urnov, Fyodor D and Jaenisch, Rudolf
Journal title
Language
English
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Scope and Contents
Contents
Targeted genetic engineering of human pluripotent cells is a prerequisite for exploiting their full potential. Such genetic manipulations can be achieved using site-specific nucleases. Here, we engineered Transcription Activation-Like Effector Nucleases (TALENs) for five distinct genomic loci. At all loci tested we obtained hESC and iPSC single-cel...
Alternative Titles
Full title
Genetic engineering of human ES and iPS cells using TALE nucleases
Authors, Artists and Contributors
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Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3152587
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3152587
Other Identifiers
ISSN
1087-0156
E-ISSN
1546-1696
DOI
10.1038/nbt.1927
