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Analysis of protein-ligand interactions by fluorescence polarization

Analysis of protein-ligand interactions by fluorescence polarization

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3160472

Analysis of protein-ligand interactions by fluorescence polarization

About this item

Full title

Analysis of protein-ligand interactions by fluorescence polarization

Author / Creator

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2011-03, Vol.6 (3), p.365-387

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Quantification of the associations between biomolecules is required both to predict and understand the interactions that underpin all biological activity. Fluorescence polarization (FP) provides a nondisruptive means of measuring the association of a fluorescent ligand with a larger molecule. We describe an FP assay in which binding of fluorescein-...

Alternative Titles

Full title

Analysis of protein-ligand interactions by fluorescence polarization

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3160472

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3160472

Other Identifiers

ISSN

1754-2189,1750-2799

E-ISSN

1750-2799

DOI

10.1038/nprot.2011.305

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