Evolution of a designed retro-aldolase leads to complete active site remodeling
Evolution of a designed retro-aldolase leads to complete active site remodeling
About this item
Full title
Author / Creator
Publisher
New York: Nature Publishing Group US
Journal title
Language
English
Formats
Publication information
Publisher
New York: Nature Publishing Group US
Subjects
More information
Scope and Contents
Contents
A previously designed enzyme used a reactive lysine to initiate cleavage of a carbon-carbon bond. Directed evolution of this construct now shows a drastic reorganization of the active site to use an alternative catalytic lysine and suggests considerations for future design efforts.
Evolutionary advances are often fueled by unanticipated innovati...
Alternative Titles
Full title
Evolution of a designed retro-aldolase leads to complete active site remodeling
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3720730
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3720730
Other Identifiers
ISSN
1552-4450
E-ISSN
1552-4469
DOI
10.1038/nchembio.1276
