Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae
Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae
About this item
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Author / Creator
Han, Hui , Zhou, Haijian , Li, Haishan , Gao, Yuan , Lu, Zhi , Hu, Kongxin and Xu, Baoliang
Publisher
Switzerland: MDPI AG
Journal title
Language
English
Formats
Publication information
Publisher
Switzerland: MDPI AG
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Scope and Contents
Contents
A total of 110 strains of Klebsiella pneumoniae were used to optimize pulsed-field gel electrophoresis (PFGE) for subtyping of K. pneumoniae. For optimization of electrophoresis parameters (EPs) of XbaI-PFGE, 11 isolates were analyzed with XbaI digestion using three EPs. The EP of a switch time of 6 to 36 s for 18.5 h gave clearest patterns and was...
Alternative Titles
Full title
Optimization of Pulse-Field Gel Electrophoresis for Subtyping of Klebsiella pneumoniae
Authors, Artists and Contributors
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Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3734452
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3734452
Other Identifiers
ISSN
1660-4601,1661-7827
E-ISSN
1660-4601
DOI
10.3390/ijerph10072720
