Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase
Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase
About this item
Full title
Author / Creator
Sanger Mouse Genetics Project , Ryder, Edward , Doe, Brendan , Gleeson, Diane , Houghton, Richard , Dalvi, Priya , Grau, Evelyn , Habib, Bishoy , Miklejewska, Evelina , Newman, Stuart , Sethi, Debarati , Sinclair, Caroline , Vyas, Sapna , Wardle-Jones, Hannah , Bottomley, Joanna , Bussell, James , Galli, Antonella , Salisbury, Jennifer and Ramirez-Solis, Ramiro
Publisher
Dordrecht: Springer-Verlag
Journal title
Language
English
Formats
Publication information
Publisher
Dordrecht: Springer-Verlag
Subjects
More information
Scope and Contents
Contents
We describe here use of a cell-permeable Cre to efficiently convert the EUCOMM/KOMP-CSD tm1a allele to the tm1b form in preimplantation mouse embryos in a high-throughput manner, consistent with the requirements of the International Mouse Phenotyping Consortium-affiliated NIH KOMP2 project. This method results in rapid allele conversion and minimiz...
Alternative Titles
Full title
Rapid conversion of EUCOMM/KOMP-CSD alleles in mouse embryos using a cell-permeable Cre recombinase
Authors, Artists and Contributors
Author / Creator
Ryder, Edward
Doe, Brendan
Gleeson, Diane
Houghton, Richard
Dalvi, Priya
Grau, Evelyn
Habib, Bishoy
Miklejewska, Evelina
Newman, Stuart
Sethi, Debarati
Sinclair, Caroline
Vyas, Sapna
Wardle-Jones, Hannah
Bottomley, Joanna
Bussell, James
Galli, Antonella
Salisbury, Jennifer
Ramirez-Solis, Ramiro
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3890051
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3890051
Other Identifiers
ISSN
0962-8819
E-ISSN
1573-9368
DOI
10.1007/s11248-013-9764-x
