Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purifi...
Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purified cDNA as Template
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New York: New York : Springer-Verlag
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English
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New York: New York : Springer-Verlag
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Contents
Quantitative real-time polymerase chain reaction (qRT-PCR) is a precise method to measure changes in gene transcript level. Accurate quantification requires careful RNA quality assessment, determination of primer efficiency, and selection of an appropriate reference gene. While many experimental procedures for these purposes have been described for...
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Evaluation of Candidate Reference Genes for Real-Time Quantitative PCR of Plant Samples Using Purified cDNA as Template
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3906740
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3906740
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ISSN
0735-9640
E-ISSN
1572-9818,1556-469X
DOI
10.1007/s11105-008-0072-1
