Highly Multiplexed Subcellular RNA Sequencing in Situ
Highly Multiplexed Subcellular RNA Sequencing in Situ
About this item
Full title
Author / Creator
Lee, Je Hyuk , Daugharthy, Evan R. , Scheiman, Jonathan , Kalhor, Reza , Yang, Joyce L. , Ferrante, Thomas C. , Terry, Richard , Jeanty, Sauveur S. F. , Li, Chao , Amamoto, Ryoji , Peters, Derek T. , Turczyk, Brian M. , Marblestone, Adam H. , Inverso, Samuel A. , Bernard, Amy , Mali, Prashant , Rios, Xavier , Aach, John and Church, George M.
Publisher
United States: American Association for the Advancement of Science
Journal title
Language
English
Formats
Publication information
Publisher
United States: American Association for the Advancement of Science
Subjects
More information
Scope and Contents
Contents
Understanding the spatial organization of gene expression with single-nucleotide resolution requires localizing the sequences of expressed RNA transcripts within a cell in situ. Here, we describe fluorescent in situ RNA sequencing (FISSEQ), in which stably cross-linked complementary DNA (cDNA) amplicons are sequenced within a biological sample. Usi...
Alternative Titles
Full title
Highly Multiplexed Subcellular RNA Sequencing in Situ
Authors, Artists and Contributors
Author / Creator
Daugharthy, Evan R.
Scheiman, Jonathan
Kalhor, Reza
Yang, Joyce L.
Ferrante, Thomas C.
Terry, Richard
Jeanty, Sauveur S. F.
Li, Chao
Amamoto, Ryoji
Peters, Derek T.
Turczyk, Brian M.
Marblestone, Adam H.
Inverso, Samuel A.
Bernard, Amy
Mali, Prashant
Rios, Xavier
Aach, John
Church, George M.
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4140943
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4140943
Other Identifiers
ISSN
0036-8075
E-ISSN
1095-9203
DOI
10.1126/science.1250212
