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A general protocol for the generation of Nanobodies for structural biology

A general protocol for the generation of Nanobodies for structural biology

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4297639

A general protocol for the generation of Nanobodies for structural biology

About this item

Full title

A general protocol for the generation of Nanobodies for structural biology

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2014-03, Vol.9 (3), p.674-693

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

There is growing interest in using antibodies as auxiliary tools to crystallize proteins. Here we describe a general protocol for the generation of Nanobodies to be used as crystallization chaperones for the structural investigation of diverse conformational states of flexible (membrane) proteins and complexes thereof. Our technology has a competit...

Alternative Titles

Full title

A general protocol for the generation of Nanobodies for structural biology

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4297639

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4297639

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/nprot.2014.039

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