Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in e...
Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays
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Publisher
England: BioMed Central Ltd
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Language
English
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Publisher
England: BioMed Central Ltd
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Contents
Highly purified nuclear protein is required when using an electrophoretic mobility shift assay (EMSA) to study transcription factors, e.g. nuclear factor-κB (NF-κB), a major transcription factor that regulates both innate and adaptive immune responses following infection. Although many protocols have been developed for nuclear protein extraction, t...
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Full title
Rapid preparation of high-purity nuclear proteins from a small number of cultured cells for use in electrophoretic mobility shift assays
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4339431
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4339431
Other Identifiers
ISSN
1471-2172
E-ISSN
1471-2172
DOI
10.1186/s12865-014-0062-z
