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An optimized method for high-titer lentivirus preparations without ultracentrifugation

An optimized method for high-titer lentivirus preparations without ultracentrifugation

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4562269

An optimized method for high-titer lentivirus preparations without ultracentrifugation

About this item

Full title

An optimized method for high-titer lentivirus preparations without ultracentrifugation

Publisher

London: Nature Publishing Group UK

Journal title

Scientific reports, 2015-09, Vol.5 (1), p.13875-13875, Article 13875

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Lentiviral technology has proven to be a powerful tool to express exogenous genes in dividing and non-dividing cells. Currently, most protocols for generating high-titer lentivirus require ultracentrifugation, which can be an instrumental barrier for routine operations in a laboratory. In this study, the effect of relative centrifugal force (RCF) o...

Alternative Titles

Full title

An optimized method for high-titer lentivirus preparations without ultracentrifugation

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4562269

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4562269

Other Identifiers

ISSN

2045-2322

E-ISSN

2045-2322

DOI

10.1038/srep13875

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