An optimized method for high-titer lentivirus preparations without ultracentrifugation
An optimized method for high-titer lentivirus preparations without ultracentrifugation
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Author / Creator
Jiang, Wei , Hua, Rui , Wei, Mengping , Li, Chenhong , Qiu, Zilong , Yang, Xiaofei and Zhang, Chen
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
Lentiviral technology has proven to be a powerful tool to express exogenous genes in dividing and non-dividing cells. Currently, most protocols for generating high-titer lentivirus require ultracentrifugation, which can be an instrumental barrier for routine operations in a laboratory. In this study, the effect of relative centrifugal force (RCF) o...
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Full title
An optimized method for high-titer lentivirus preparations without ultracentrifugation
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Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4562269
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4562269
Other Identifiers
ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/srep13875