Determining exon connectivity in complex mRNAs by nanopore sequencing
Determining exon connectivity in complex mRNAs by nanopore sequencing
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Publisher
England: BioMed Central Ltd
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Language
English
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Publisher
England: BioMed Central Ltd
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Scope and Contents
Contents
Short-read high-throughput RNA sequencing, though powerful, is limited in its ability to directly measure exon connectivity in mRNAs that contain multiple alternative exons located farther apart than the maximum read length. Here, we use the Oxford Nanopore MinION sequencer to identify 7,899 'full-length' isoforms expressed from four Drosophila gen...
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Full title
Determining exon connectivity in complex mRNAs by nanopore sequencing
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4588896
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4588896
Other Identifiers
ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-015-0777-z
