Proteome-wide enrichment of proteins modified by lysine methylation
Proteome-wide enrichment of proteins modified by lysine methylation
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Publisher
London: Nature Publishing Group UK
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English
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Publisher
London: Nature Publishing Group UK
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Scope and Contents
Contents
We present a protocol for using the triple malignant brain tumor domains of L3MBTL1 (3xMBT), which bind to mono- and di-methylated lysine with minimal sequence specificity, in order to enrich for such methylated lysine from cell lysates. Cells in culture are grown with amino acids containing light or heavy stable isotopic labels. Methylated protein...
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Full title
Proteome-wide enrichment of proteins modified by lysine methylation
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4651005
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4651005
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2013.164
