Target engagement and drug residence time can be observed in living cells with BRET
Target engagement and drug residence time can be observed in living cells with BRET
About this item
Full title
Author / Creator
Robers, Matthew B. , Dart, Melanie L. , Woodroofe, Carolyn C. , Zimprich, Chad A. , Kirkland, Thomas A. , Machleidt, Thomas , Kupcho, Kevin R. , Levin, Sergiy , Hartnett, James R. , Zimmerman, Kristopher , Niles, Andrew L. , Ohana, Rachel Friedman , Daniels, Danette L. , Slater, Michael , Wood, Monika G. , Cong, Mei , Cheng, Yi-Qiang and Wood, Keith V.
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
The therapeutic action of drugs is predicated on their physical engagement with cellular targets. Here we describe a broadly applicable method using bioluminescence resonance energy transfer (BRET) to reveal the binding characteristics of a drug with selected targets within intact cells. Cell-permeable fluorescent tracers are used in a competitive...
Alternative Titles
Full title
Target engagement and drug residence time can be observed in living cells with BRET
Authors, Artists and Contributors
Author / Creator
Dart, Melanie L.
Woodroofe, Carolyn C.
Zimprich, Chad A.
Kirkland, Thomas A.
Machleidt, Thomas
Kupcho, Kevin R.
Levin, Sergiy
Hartnett, James R.
Zimmerman, Kristopher
Niles, Andrew L.
Ohana, Rachel Friedman
Daniels, Danette L.
Slater, Michael
Wood, Monika G.
Cong, Mei
Cheng, Yi-Qiang
Wood, Keith V.
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4686764
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4686764
Other Identifiers
ISSN
2041-1723
E-ISSN
2041-1723
DOI
10.1038/ncomms10091
