CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries
CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries
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England: BioMed Central Ltd
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Language
English
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England: BioMed Central Ltd
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Genetic screens using CRISPR/Cas9 are a powerful method for the functional analysis of genomes.
Here we describe CRISPR library designer (CLD), an integrated bioinformatics application for the design of custom single guide RNA (sgRNA) libraries for all organisms with annotated genomes. CLD is suitable for the design of libraries using modified CRISPR enzymes and targeting non-coding regions. To demonstrate its utility, we perform a pooled screen for modulators of the TNF-related apoptosis inducing ligand (TRAIL) pathway using a custom library of 12,471 sgRNAs.
CLD predicts a high fraction of functional sgRNAs and is publicly available at https://github.com/boutroslab/cld....
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CRISPR library designer (CLD): software for multispecies design of single guide RNA libraries
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4807595
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4807595
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ISSN
1474-760X,1474-7596
E-ISSN
1474-760X
DOI
10.1186/s13059-016-0915-2
