biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
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United States: National Academy of Sciences
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Language
English
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Publisher
United States: National Academy of Sciences
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Contents
Analyses of protein complexes are facilitated by methods that enable the generation of recombinant complexes via coexpression of their subunits from multigene DNA constructs. However, low experimental throughput limits the generation of such constructs in parallel. Here we describe a method that allows up to 25 cDNAs to be assembled into a single b...
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Full title
biGBac enables rapid gene assembly for the expression of large multisubunit protein complexes
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4868461
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4868461
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.1604935113
