Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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Beyond gene editing, the CRISPR–Cas9 technology offers a versatile sequence-specific gene regulation 'toolset', by utilizing the nuclease-deficient dCas9, which was designed not to cleave DNA but to precisely and specifically bind DNA when guided by a single guide RNA (sgRNA).
In CRISPR interference (CRISPRi), dCas9 is targeted to...
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Full title
Beyond editing: repurposing CRISPR–Cas9 for precision genome regulation and interrogation
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4922510
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4922510
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ISSN
1471-0072
E-ISSN
1471-0080
DOI
10.1038/nrm.2015.2
