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Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning...

Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5025511

Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic images

About this item

Full title

Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic images

Publisher

Cham: Springer International Publishing

Journal title

Advanced structural and chemical imaging, 2016-09, Vol.2 (1), p.11-13, Article 11

Language

English

Formats

Publication information

Publisher

Cham: Springer International Publishing

More information

Scope and Contents

Contents

Serial block-face scanning electron microscopy (SBEM) is quickly becoming an important imaging tool to explore three-dimensional biological structure across spatial scales. At probe-beam-electron energies of 2.0 keV or lower, the axial resolution should improve, because there is less primary electron penetration into the block face. More specifical...

Alternative Titles

Full title

Deceleration of probe beam by stage bias potential improves resolution of serial block-face scanning electron microscopic images

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5025511

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5025511

Other Identifiers

ISSN

2198-0926

E-ISSN

2198-0926

DOI

10.1186/s40679-016-0025-y

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