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Sequencing of first-strand cDNA library reveals full-length transcriptomes

Sequencing of first-strand cDNA library reveals full-length transcriptomes

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5054741

Sequencing of first-strand cDNA library reveals full-length transcriptomes

About this item

Full title

Sequencing of first-strand cDNA library reveals full-length transcriptomes

Publisher

London: Nature Publishing Group UK

Journal title

Nature communications, 2015-01, Vol.6 (1), p.6002-6002, Article 6002

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Massively parallel strand-specific sequencing of RNA (ssRNA-seq) has emerged as a powerful tool for profiling complex transcriptomes. However, many current methods for ssRNA-seq suffer from the underrepresentation of both the 5′ and 3′ ends of RNAs, which can be attributed to second-strand cDNA synthesis. The 5′ and 3′ ends of RNA harbour crucial i...

Alternative Titles

Full title

Sequencing of first-strand cDNA library reveals full-length transcriptomes

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5054741

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5054741

Other Identifiers

ISSN

2041-1723

E-ISSN

2041-1723

DOI

10.1038/ncomms7002

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