Vasopressin-mediated regulation of renal water excretion is defective in a variety of water balance disorders in humans. It occurs in part through long-term mechanisms that regulate the abundance of the aquaporin-2 water channel in renal collecting duct cells. Here, we use deep DNA sequencing in mouse collecting duct cells to ask whether vasopressin signaling selectively increases
Aqp2
gene transcription or whether it triggers a broadly targeted transcriptional network. ChIP-Seq quantification of binding sites for RNA polymerase II was combined with RNA-Seq quantification of transcript abundances to identify genes whose transcription is regulated by vasopressin. (View curated dataset at
https://helixweb.nih.gov/ESBL/Database/Vasopressin/
). The analysis revealed only 35 vasopressin-regulated genes (of 3659) including
Aqp2
. Increases in RNA polymerase II binding and mRNA abundances for
Aqp2
far outstripped corresponding measurements for all other genes, consistent with the conclusion that vasopressin-mediated transcriptional regulation is highly selective for

Systems-level analysis reveals selective regulation of Aqp2 gene expression by vasopressin

10.1038/srep34863