Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides inte...
Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides internalization pathways: optimization, pitfalls, comparison with mass spectrometry quantification
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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The mechanism of cell-penetrating peptides entry into cells is unclear, preventing the development of more efficient vectors for biotechnological or therapeutic purposes. Here, we developed a protocol relying on fluorometry to distinguish endocytosis from direct membrane translocation, using Penetratin, TAT and R9. The quantities of internalized CP...
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Quantitative fluorescence spectroscopy and flow cytometry analyses of cell-penetrating peptides internalization pathways: optimization, pitfalls, comparison with mass spectrometry quantification
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5107916
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5107916
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/srep36938
