Augmenting CRISPR applications in Drosophila with tRNA-flanked Cas9 and Cpf1 sgRNAs
Augmenting CRISPR applications in Drosophila with tRNA-flanked Cas9 and Cpf1 sgRNAs
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English
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We present tRNA-based vectors for producing multiple clustered regularly-interspaced short palindromic repeats (CRISPR) sgRNAs from a single RNA polymerase II or III transcript in
Drosophila
. The system, which is based on liberation of sgRNAs by processing of flanking tRNAs, permits highly efficient multiplexing of Cas9-based mutagenesis. We...
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Full title
Augmenting CRISPR applications in Drosophila with tRNA-flanked Cas9 and Cpf1 sgRNAs
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5215823
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5215823
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ISSN
1548-7091
E-ISSN
1548-7105
DOI
10.1038/nmeth.3972
