Short DNA Hairpins Compromise Recombinant Adeno-Associated Virus Genome Homogeneity
Short DNA Hairpins Compromise Recombinant Adeno-Associated Virus Genome Homogeneity
About this item
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Author / Creator
Xie, Jun , Mao, Qin , Tai, Phillip W.L. , He, Ran , Ai, Jianzhong , Su, Qin , Zhu, Ye , Ma, Hong , Li, Jia , Gong, Shoufang , Wang, Dan , Gao, Zhen , Li, Mengxin , Zhong, Li , Zhou, Heather and Gao, Guangping
Publisher
United States: Elsevier Inc
Journal title
Language
English
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Publication information
Publisher
United States: Elsevier Inc
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More information
Scope and Contents
Contents
Short hairpin (sh)RNAs delivered by recombinant adeno-associated viruses (rAAVs) are valuable tools to study gene function in vivo and a promising gene therapy platform. Our data show that incorporation of shRNA transgenes into rAAV constructs reduces vector yield and produces a population of truncated and defective genomes. We demonstrate that seq...
Alternative Titles
Full title
Short DNA Hairpins Compromise Recombinant Adeno-Associated Virus Genome Homogeneity
Authors, Artists and Contributors
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5474962
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5474962
Other Identifiers
ISSN
1525-0016
E-ISSN
1525-0024
DOI
10.1016/j.ymthe.2017.03.028
