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Plasmid-based one-pot saturation mutagenesis

Plasmid-based one-pot saturation mutagenesis

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5666567

Plasmid-based one-pot saturation mutagenesis

About this item

Full title

Plasmid-based one-pot saturation mutagenesis

Publisher

New York: Nature Publishing Group US

Journal title

Nature methods, 2016-11, Vol.13 (11), p.928-930

Language

English

Formats

Publication information

Publisher

New York: Nature Publishing Group US

More information

Scope and Contents

Contents

Nicking mutagenesis is a streamlined protocol for plasmid-based single-pot comprehensive saturation mutagenesis.
Deep mutational scanning is a foundational tool for addressing the functional consequences of large numbers of mutants, but a more efficient and accessible method for construction of user-defined mutagenesis libraries is needed. Here...

Alternative Titles

Full title

Plasmid-based one-pot saturation mutagenesis

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5666567

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5666567

Other Identifiers

ISSN

1548-7091

E-ISSN

1548-7105

DOI

10.1038/nmeth.4029

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