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Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pre...

Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pre...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5883035

Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure

About this item

Full title

Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure

Publisher

London: Nature Publishing Group UK

Journal title

Scientific reports, 2018-04, Vol.8 (1), p.5467-11, Article 5467

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Extraction of cell-free DNA (cfDNA), which exists at an extremely low concentration in plasma, is a critical process for either targeted-sensing or massive sequencing of DNAs. However, such small amount of DNA cannot be fully obtained without high-speed centrifugation (<20,000 g). Here, we developed a centrifugation-free cfDNA extraction method and...

Alternative Titles

Full title

Centrifugation-free extraction of circulating nucleic acids using immiscible liquid under vacuum pressure

Authors, Artists and Contributors

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5883035

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5883035

Other Identifiers

ISSN

2045-2322

E-ISSN

2045-2322

DOI

10.1038/s41598-018-23766-9

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