Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM
Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM
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Author / Creator
Jia, Yu , Xu, Rong-Gang , Ren, Xingjie , Ewen-Campen, Ben , Rajakumar, Rajendhran , Zirin, Jonathan , Yang-Zhou, Donghui , Zhu, Ruibao , Wang, Fang , Mao, Decai , Peng, Ping , Qiao, Huan-Huan , Wang, Xia , Liu, Lu-Ping , Xu, Bowen , Ji, Jun-Yuan , Liu, Qingfei , Sun, Jin , Perrimon, Norbert and Ni, Jian-Quan
Publisher
United States: National Academy of Sciences
Journal title
Language
English
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Publisher
United States: National Academy of Sciences
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More information
Scope and Contents
Contents
CRISPR/Cas9-based transcriptional activation (CRISPRa) has recently emerged as a powerful and scalable technique for systematic overexpression genetic analysis in Drosophila melanogaster. We present flySAM, a potent tool for in vivo CRISPRa, which offers major improvements over existing strategies in terms of effectiveness, scalability, and ease of...
Alternative Titles
Full title
Next-generation CRISPR/Cas9 transcriptional activation in Drosophila using flySAM
Authors, Artists and Contributors
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Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5939105
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5939105
Other Identifiers
ISSN
0027-8424
E-ISSN
1091-6490
DOI
10.1073/pnas.1800677115
