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Global site-specific analysis of glycoprotein N-glycan processing

Global site-specific analysis of glycoprotein N-glycan processing

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5941933

Global site-specific analysis of glycoprotein N-glycan processing

About this item

Full title

Global site-specific analysis of glycoprotein N-glycan processing

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2018-06, Vol.13 (6), p.1196-1212

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

This protocol describes a semiquantitative glycoproteomics method using sequential treatment with endoglycosidases to create unique mass signatures to determine glycan occupancy and proportion of high-mannose and complex glycans at each glycosite.
N-glycans contribute to the folding, stability and functions of the proteins they decorate. They ar...

Alternative Titles

Full title

Global site-specific analysis of glycoprotein N-glycan processing

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5941933

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5941933

Other Identifiers

ISSN

1754-2189

E-ISSN

1750-2799

DOI

10.1038/nprot.2018.024

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