Global site-specific analysis of glycoprotein N-glycan processing
Global site-specific analysis of glycoprotein N-glycan processing
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
This protocol describes a semiquantitative glycoproteomics method using sequential treatment with endoglycosidases to create unique mass signatures to determine glycan occupancy and proportion of high-mannose and complex glycans at each glycosite.
N-glycans contribute to the folding, stability and functions of the proteins they decorate. They ar...
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Global site-specific analysis of glycoprotein N-glycan processing
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5941933
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5941933
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ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/nprot.2018.024