CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method
CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method
About this item
Full title
Author / Creator
Wang, Qiao , Zhang, Beibei , Xu, Xinhui , Long, Feifei and Wang, Jinke
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
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Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
This study develops a new method for detecting and typing target DNA based on Cas9 nuclease, which was named as ctPCR, representing Cas9-sgRNA- or CRISPR-typing PCR. The technique can detect and type target DNA easily, rapidly, specifically, and sensitively. This technique detects target DNA in three steps: (1) amplifying target DNA with PCR by usi...
Alternative Titles
Full title
CRISPR-typing PCR (ctPCR), a new Cas9-based DNA detection method
Authors, Artists and Contributors
Author / Creator
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Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6148268
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6148268
Other Identifiers
ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-018-32329-x
