Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tiss...
Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry
About this item
Full title
Author / Creator
Pacific Northwest National Lab. (PNNL), Richland, WA (United States) , Mertins, Philipp , Tang, Lauren C. , Krug, Karsten , Clark, David J. , Gritsenko, Marina A. , Chen, Lijun , Clauser, Karl R. , Clauss, Therese R. , Shah, Punit , Gillette, Michael A. , Petyuk, Vladislav A. , Thomas, Stefani N. , Mani, D. R. , Mundt, Filip , Moore, Ronald J. , Hu, Yingwei , Zhao, Rui , Schnaubelt, Michael , Keshishian, Hasmik , Monroe, Matthew E. , Zhang, Zhen , Udeshi, Namrata D. , Mani, Deepak , Davies, Sherri R. , Townsend, R. Reid , Chan, Daniel W. , Smith, Richard D. , Zhang, Hui , Liu, Tao and Carr, Steven A.
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
More information
Scope and Contents
Contents
Here we present an optimized workflow for global proteome and phosphoproteome analysis of tissues or cell lines that uses isobaric tags (TMT (tandem mass tags)-10) for multiplexed analysis and relative quantification, and provides 3× higher throughput than iTRAQ (isobaric tags for absolute and relative quantification)-4-based methods with high intr...
Alternative Titles
Full title
Reproducible workflow for multiplexed deep-scale proteome and phosphoproteome analysis of tumor tissues by liquid chromatography–mass spectrometry
Authors, Artists and Contributors
Author / Creator
Mertins, Philipp
Tang, Lauren C.
Krug, Karsten
Clark, David J.
Gritsenko, Marina A.
Chen, Lijun
Clauser, Karl R.
Clauss, Therese R.
Shah, Punit
Gillette, Michael A.
Petyuk, Vladislav A.
Thomas, Stefani N.
Mani, D. R.
Mundt, Filip
Moore, Ronald J.
Hu, Yingwei
Zhao, Rui
Schnaubelt, Michael
Keshishian, Hasmik
Monroe, Matthew E.
Zhang, Zhen
Udeshi, Namrata D.
Mani, Deepak
Davies, Sherri R.
Townsend, R. Reid
Chan, Daniel W.
Smith, Richard D.
Zhang, Hui
Liu, Tao
Carr, Steven A.
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6211289
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6211289
Other Identifiers
ISSN
1754-2189
E-ISSN
1750-2799
DOI
10.1038/s41596-018-0006-9
