Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient In...
Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins
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Publisher
Switzerland: MDPI AG
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Language
English
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Switzerland: MDPI AG
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Cell-free protein synthesis is useful for synthesizing difficult targets. The site-specific incorporation of non-natural amino acids into proteins is a powerful protein engineering method. In this study, we optimized the protocol for cell extract preparation from the Escherichia coli strain RFzero-iy, which is engineered to lack release factor 1 (R...
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Cell-Free Protein Synthesis Using S30 Extracts from Escherichia coli RFzero Strains for Efficient Incorporation of Non-Natural Amino Acids into Proteins
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6387211
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6387211
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ISSN
1422-0067,1661-6596
E-ISSN
1422-0067
DOI
10.3390/ijms20030492
