Utilizing a Simple Method for Stoichiometric Protein Labeling to Quantify Antibody Blockade
Utilizing a Simple Method for Stoichiometric Protein Labeling to Quantify Antibody Blockade
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
Ligand binding assays routinely employ fluorescently-labeled protein ligands to quantify the extent of binding. These ligands are commonly generated through chemical modification of accessible lysine residues, which often results in heterogeneous populations exhibiting variable binding properties. This could be remedied by quantitative, site-specif...
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Full title
Utilizing a Simple Method for Stoichiometric Protein Labeling to Quantify Antibody Blockade
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6504924
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6504924
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-019-43469-z
