Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroetha...
Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroethanol
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Publisher
London: Nature Publishing Group UK
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Language
English
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London: Nature Publishing Group UK
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Contents
The incorporation of 2,2,2-trichloroethanol in polyacrylamide gels allows for fluorescent visualization of proteins following electrophoresis. Ultraviolet-light exposure, in the presence of this trichlorinated compound, results in a covalent modification of the tryptophan indole ring that shifts the fluorescent emission into the visible range. Base...
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Full title
Protein quantification and visualization via ultraviolet-dependent labeling with 2,2,2-trichloroethanol
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6763483
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6763483
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-019-50385-9
