Despite the use of
Hymenolepis diminuta
as a model organism in experimental parasitology, a full genome description has not yet been published. Here we present a hybrid
de novo
genome assembly based on complementary sequencing technologies and methods. The combination of Illumina paired-end, Illumina mate-pair and Oxford Nanopore Technology reads greatly improved the assembly of the
H
.
diminuta
genome. Our results indicate that the hybrid sequencing approach is the method of choice for obtaining high-quality data. The final genome assembly is 177 Mbp with contig N50 size of 75 kbp and a scaffold N50 size of 2.3 Mbp. We obtained one of the most complete cestode genome assemblies and annotated 15,169 potential protein-coding genes. The obtained data may help explain cestode gene function and better clarify the evolution of its gene families, and thus the adaptive features evolved during millennia of co-evolution with their hosts.
Measurement(s)
whole genome sequencing assay • transcription profiling assay • sequence_assembly • sequence annotation • mitochondrial DNA
Technology Type(s)
DNA sequencing • RNA sequencing • genome assembly • bioinformatics analysis
Sample Characteristic - Organism
Hymenolepis diminuta
Machine-accessible metadata file describing the reported data:
https://doi.org/10.6084/m9.figshare.10283018...

Hybrid de novo whole-genome assembly and annotation of the model tapeworm Hymenolepis diminuta

10.1038/s41597-019-0311-3