Characterization of a human liver reference material fit for proteomics applications
Characterization of a human liver reference material fit for proteomics applications
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Full title
Author / Creator
Publisher
London: Nature Publishing Group UK
Journal title
Language
English
Formats
Publication information
Publisher
London: Nature Publishing Group UK
Subjects
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Scope and Contents
Contents
The National Institute of Standards and Technology (NIST) is creating new, economical, qualitative reference materials and data for proteomics comparisons, benchmarking and harmonization. Here we describe a large dataset from shotgun proteomic analysis of RM 8461 Human Liver for Proteomics, a reference material being developed. Consensus identifications using multiple search engines and sample preparations demonstrate a homogeneous and fit-for-purpose material that can be incorporated into automated or manual sample preparation workflows, with the resulting data used to directly assess complete sample-to-data workflows and provide harmonization and benchmarking between laboratories and techniques. Data are available via PRIDE with identifier PXD013608.
Measurement(s)
peptide sequence-level identification attribute • protein expression profiling
Technology Type(s)
liquid chromatography-tandem mass spectrometry
Factor Type(s)
mass of liver sample
Sample Characteristic - Organism
Homo sapiens
Machine-accessible metadata file describing the reported data:
https://doi.org/10.6084/m9.figshare.11310485...
Alternative Titles
Full title
Characterization of a human liver reference material fit for proteomics applications
Authors, Artists and Contributors
Author / Creator
Identifiers
Primary Identifiers
Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6920408
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_6920408
Other Identifiers
ISSN
2052-4463
E-ISSN
2052-4463
DOI
10.1038/s41597-019-0336-7
