Streptomyces
are efficient producers of various bioactive compounds, which are mostly synthesized by their secondary metabolite biosynthetic gene clusters (smBGCs). The smBGCs are tightly controlled by complex regulatory systems at transcriptional and translational levels to effectively utilize precursors that are supplied by primary metabolism. Thus, dynamic changes in gene expression in response to cellular status at both the transcriptional and translational levels should be elucidated to directly reflect protein levels, rapid downstream responses, and cellular energy costs. In this study, RNA-Seq and ribosome profiling were performed for five industrially important
Streptomyces
species at different growth phases, for the deep sequencing of total mRNA, and only those mRNA fragments that are protected by translating ribosomes, respectively. Herein, 12.0 to 763.8 million raw reads were sufficiently obtained with high quality of more than 80% for the Phred score Q30 and high reproducibility. These data provide a comprehensive understanding of the transcriptional and translational landscape across the
Streptomyces
species and contribute to facilitating the rational engineering of secondary metabolite production.
Measurement(s)
transcriptome • translation • translatome
Technology Type(s)
RNA sequencing • Ribo-Seq
Factor Type(s)
Growth phases
Sample Characteristic - Organism
Streptomyces avermitilis • Streptomyces clavuligerus • Streptomyces lividans • Streptomyces venezuelae • Streptomyces tsukubensis
Machine-accessible metadata file describing the reported data:
https://doi.org/10.6084/m9.figshare.12045603...

Transcriptome and translatome profiles of Streptomyces species in different growth phases

10.1038/s41597-020-0476-9