Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
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Publisher
London: Nature Publishing Group UK
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Language
English
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Publisher
London: Nature Publishing Group UK
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Contents
CRISPR/Cas-mediated genome editing is a powerful tool for generating genetically mutated cells and organisms. Linearisation of donor cassettes with this system has been shown to facilitate both transgene donor insertion and targeted knock-in. Here, we developed a donor plasmid that we name pCriMGET (
p
lasmid of synthetic
CRI
SPR coded...
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Full title
Genome editing with the donor plasmid equipped with synthetic crRNA-target sequence
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7445171
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7445171
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-020-70804-6
