Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of...
Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS
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London: Nature Publishing Group UK
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English
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London: Nature Publishing Group UK
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In recent years, advanced HPLC-MS strategies based on intact protein (“top-down”) or protein subunit (“middle-up/middle-down”) analysis have been implemented for the characterization of therapeutic monoclonal antibodies. Here, we assess feasibility of middle-up/middle-down analysis for polyclonal IgGs exhibiting extensive sequence variability. Spec...
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Towards middle-up analysis of polyclonal antibodies: subclass-specific N-glycosylation profiling of murine immunoglobulin G (IgG) by means of HPLC-MS
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TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7581757
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https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7581757
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ISSN
2045-2322
E-ISSN
2045-2322
DOI
10.1038/s41598-020-75045-1
