A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures
A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures
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Author / Creator
Publisher
United States: John Wiley & Sons, Inc
Journal title
Language
English
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Publication information
Publisher
United States: John Wiley & Sons, Inc
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Scope and Contents
Contents
Background
CRISPR‐Cas9‐based technologies have revolutionized experimental manipulation of mammalian genomes. None‐the‐less, limitations of the delivery and efficacy of these technologies restrict their application in primary cells.
Aims
To create an optimized protocol for penetrant, reproducible, and fast targeted genome editing in cell c...
Alternative Titles
Full title
A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures
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Record Identifier
TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7685144
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7685144
Other Identifiers
ISSN
2573-8348
E-ISSN
2573-8348
DOI
10.1002/cnr2.1269