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A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures

A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7685144

A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures

About this item

Full title

A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures

Publisher

United States: John Wiley & Sons, Inc

Journal title

Cancer reports, 2020-10, Vol.3 (5), p.e1269-n/a

Language

English

Formats

Publication information

Publisher

United States: John Wiley & Sons, Inc

More information

Scope and Contents

Contents

Background
CRISPR‐Cas9‐based technologies have revolutionized experimental manipulation of mammalian genomes. None‐the‐less, limitations of the delivery and efficacy of these technologies restrict their application in primary cells.
Aims
To create an optimized protocol for penetrant, reproducible, and fast targeted genome editing in cell c...

Alternative Titles

Full title

A simple and highly efficient method for multi‐allelic CRISPR‐Cas9 editing in primary cell cultures

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7685144

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7685144

Other Identifiers

ISSN

2573-8348

E-ISSN

2573-8348

DOI

10.1002/cnr2.1269

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