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High-resolution in situ structure determination by cryo-electron tomography and subtomogram averagin...

High-resolution in situ structure determination by cryo-electron tomography and subtomogram averagin...

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9251519

High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity

About this item

Full title

High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity

Publisher

London: Nature Publishing Group UK

Journal title

Nature protocols, 2022-02, Vol.17 (2), p.421-444

Language

English

Formats

Publication information

Publisher

London: Nature Publishing Group UK

More information

Scope and Contents

Contents

Cryo-electron tomography and subtomogram averaging (STA) has developed rapidly in recent years. It provides structures of macromolecular complexes in situ and in cellular context at or below subnanometer resolution and has led to unprecedented insights into the inner working of molecular machines in their native environment, as well as their functi...

Alternative Titles

Full title

High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity

Identifiers

Primary Identifiers

Record Identifier

TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9251519

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_9251519

Other Identifiers

ISSN

1754-2189,1750-2799

E-ISSN

1750-2799

DOI

10.1038/s41596-021-00648-5

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