RP-HPLC法测定垫状卷柏中穗花杉双黄酮的含量
RP-HPLC法测定垫状卷柏中穗花杉双黄酮的含量
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Full title
RP-HPLC法测定垫状卷柏中穗花杉双黄酮的含量
Author / Creator
Publisher
河南大学天然药物研究所,开封,475004
Journal title
Tian ran chan wu yan jiu yu kai fa, 2008, Vol.20 (5), p.863-865
Language
Chinese
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Publisher
河南大学天然药物研究所,开封,475004
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Scope and Contents
Contents
本文用反相高效液相色谱法测定垫状卷柏药材中的穗花杉双黄酮。选用Purospher star RP-C18色谱柱（250mm×4．6mm，5μm），以甲醇-0．1％磷酸水溶液（65：35）为流动相，流速为O．8mL／min，检测波长337nm，柱温25℃进行检测。结果显示，穗花杉双黄酮的含量在0．038—0．342μg范围内时进样量与峰面积积分值呈良好的线性关系，r=0．9999，平均回收率为101．2％，RSD为2．3％（n=6）。该方法测定垫状卷柏药材中的穗花杉双黄酮含量灵敏、准确、重现性好。
Alternative Titles
Full title
RP-HPLC法测定垫状卷柏中穗花杉双黄酮的含量
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Author / Creator
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Record Identifier
TN_cdi_wanfang_journals_trcwyjykf200805026
Permalink
https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_trcwyjykf200805026
Other Identifiers
ISSN
1001-6880
DOI
10.3969/j.issn.1001-6880.2008.05.026
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