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DC-CIK细胞的生物学活性及抗淋巴瘤细胞的作用

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DC-CIK细胞的生物学活性及抗淋巴瘤细胞的作用

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_xaykdxxb200703015

DC-CIK细胞的生物学活性及抗淋巴瘤细胞的作用

About this item

Full title

DC-CIK细胞的生物学活性及抗淋巴瘤细胞的作用

Author / Creator

魏绪仓 , 连小云 , 赵文理 , 杨娣娣 , 韩秀蕊 , 邢佩霓 and 李梅生

Publisher

陕西省人民医院血液病研究室,陕西西安,710068

Journal title

西安交通大学学报（医学版）, 2007, Vol.28 (3), p.283-318

Language

Chinese

Formats

Articles

Publication information

Publisher

陕西省人民医院血液病研究室,陕西西安,710068

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Scope and Contents

Contents

R733.7; 目的 研究细胞因子诱导的杀伤细胞(CIK)与树突状细胞(DC)共培养后DC-CIK细胞的体外增殖能力、免疫表型变化、分泌细胞因子水平以及抗淋巴瘤细胞活性.方法 正常人外周血单个核细胞诱导DC和CIK细胞,将DC与CIK共培养,以CIK细胞单独培养为对照.用台盼蓝活细胞计数计算细胞扩增倍数,MTT法测定杀伤活性,流式细胞术分析免疫表型,ELISA双抗体夹心法检测分泌干扰素-γ(IFN-γ)、白细胞介素-12(IL-12)的水平.结果 DC-CIK细胞增殖能力明显高于CIK细胞(P＜0.05);DC、CIK细胞共培养后,CD3+CD8+、CD3+CD56+双阳性细胞比率较同条件下CIK细胞组显著增多(P＜0.05);共培养3 d,DC-CIK细胞上清液中IL-12、INF-γ的分...

Alternative Titles

Full title

DC-CIK细胞的生物学活性及抗淋巴瘤细胞的作用

Authors, Artists and Contributors

Author / Creator

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Primary Identifiers

Record Identifier

TN_cdi_wanfang_journals_xaykdxxb200703015

Permalink

https://devfeature-collection.sl.nsw.gov.au/record/TN_cdi_wanfang_journals_xaykdxxb200703015

Other Identifiers

ISSN

1671-8259

DOI

10.3969/j.issn.1671-8259.2007.03.015

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